Method of long-term central venous catheterization in pigs for research

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This method of catheterization and catheter care allows the realization of long-term experiments with comfortable and stress-free blood sampling” Klein et al 92019).

Abstract:

INTRODUCTION: The pig is one of the most valuable in vivo models in biomedical research, however with only a few well-accessible veins suitable for venipuncture. Moreover, most of the known methods of blood collection are suitable only for a limited time period. The aim of the study was to verify an improved method of long-term catheterization of the jugular vein in pigs.

METHODS: A 420 mm polyurethane catheter 16G tube was surgically inserted using the Seldinger technique. The part of the tube that was not inserted into the vein was threaded through a subcutaneously introduced trocar into the occipital area, where it was well accessible and well protected from damage. The catheters were flushed with sterile 0.9% saline solution and locked with 4% citrate between frequent blood samplings, or with 30% citrate at intervals of 1-2-days. Once a week, the catheters were locked with 4% citrate containing taurolidine for 24 h in order to prevent infection. The method was verified in 14 pigs.

RESULTS: The catheters were fully functional for up to 11 weeks and no infection or thrombus was observed.

DISCUSSION: This method of catheterization and catheter care allows the realization of long-term experiments with comfortable and stress-free blood sampling.

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Reference:

Klein, P., Nalos, L., Dejmek, J. and Soukup, M. (2019) The method of long-term catheterization of the vena jugularis in pigs. Journal of Pharmacological and Toxicological Methods. May 14th. . doi: 10.1016/j.vascn.2019.106584.

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