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Blood culture bottle fill volume – Full Text

"Therefore, it is crucial to fill a blood culture bottle with enough blood to maximize the detection of bacteremia. Current CLSI guidelines for adult blood culture collection recommend 2 sets, that is, 4 bottles of blood cultures, each filled with 10 mL of blood to maximize detection of bacteremia" Keller et al (2024).

"Therefore, it is crucial to fill a blood culture bottle with enough blood to maximize the detection of bacteremia. Current CLSI guidelines for adult blood culture collection recommend 2 sets, that is, 4 bottles of blood cultures, each filled with 10 mL of blood to maximize detection of bacteremia" Keller et al (2024).

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Blood culture bottle fill volume

Abstract:

“Therefore, it is crucial to fill a blood culture bottle with enough blood to maximize the detection of bacteremia. Current CLSI guidelines for adult blood culture collection recommend 2 sets, that is, 4 bottles of blood cultures, each filled with 10 mL of blood to maximize detection of bacteremia. In practice, it is common to find poor bottle fill with many in the medical literature reporting that bottle fills less than 5 cc is a common problem.”

Reference:

Keller M, Nguyen T, Palazzo J, Lawton K, Landers M, Hyatt S, Chaturvedi V. “Wine”ing about blood culture bottles: using easy-to-follow visual cues to collect appropriate volumes. Infect Control Hosp Epidemiol. 2024 Mar 15:1-2. doi: 10.1017/ice.2024.42. Epub ahead of print. PMID: 38487825.

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